Journal: Cancer Management and Research

Article Title:

LncRNA EMX2OS Induces Proliferation, Invasion and Sphere Formation of Ovarian Cancer Cells via Regulating the miR-654-3p/AKT3/PD-L1 Axis

doi: 10.2147/cmar.s229013

Figure Lengend Snippet: Figure 7 EMX2OS promoted OC cell proliferation, invasion and sphere formation via regulating the AKT3/PD-L1 axis by sponging miR-654. (A) Western blot analysis of PD-L1 in OC cell lines and normal ovary cell IOSE-80. (B) Western blot analysis of PD-L1 in ES-2 cells transfected with AKT3 siRNAs or control siRNA, and in SKOV-3 cells transfected with AKT3 vector or control vector. (C, F, I) Proliferation of ES-2 cells transfected with EMX2OS siRNA (C), miR-654 mimic (F) or AKT3 siRNA (I), together with (or without) PD-L1 vector. (D, G, J) Invasion of ES-2 cells transfected with EMX2OS siRNA (D), miR-654 mimic (G) or AKT3 siRNA (J), together with (or without) PD-L1 vector. Scale bar: 50 μm in magnification × 100. (E, H, K) Sphere formation ES-2 cells transfected with EMX2OS siRNA (E), miR-654 mimic (H) or AKT3 siRNA (K), together with (or without) PD-L1 vector. Scale bar: 50 μm in magnification × 100. *P<0.05. Abbreviation: OC, ovarian cancer.

Article Snippet: 44 .3 3 on 1 2- A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 primary antibody against AKT3 (1:1000, Santa Cruz Biotech, Santa Cruz, CA), PD-L1 (1:1000, Proteintech, Chicago, IL, USA) and GAPDH (1:1000, Santa Cruz Biotech, Santa Cruz, CA).

Techniques: Western Blot, Transfection, Control, Plasmid Preparation

Journal: Cancer Management and Research

Article Title:

LncRNA EMX2OS Induces Proliferation, Invasion and Sphere Formation of Ovarian Cancer Cells via Regulating the miR-654-3p/AKT3/PD-L1 Axis

doi: 10.2147/cmar.s229013

Figure Lengend Snippet: Figure 8 PD-L1 is overexpressed in OC and high PD-L1 expression was correlated with poor prognosis of OC patients. (A) qRT-PCR analysis PD-L1 expression of in OC samples (n = 50) and normal ovarian tissues (n = 50). *P<0.05. (B) Kaplan–Meier curves for overall survival were created using the Kaplan–Meier Plotter with OC patients classified according to high and low PD-L1 expression. (C) Schema illustrating the mechanism by which EMX2OS induces proliferation, invasion and sphere formation of ovarian cancer cells via regulating the miR-654-3p/AKT3/PD-L1 axis. Abbreviation: OC, ovarian cancer.

Article Snippet: 44 .3 3 on 1 2- A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 primary antibody against AKT3 (1:1000, Santa Cruz Biotech, Santa Cruz, CA), PD-L1 (1:1000, Proteintech, Chicago, IL, USA) and GAPDH (1:1000, Santa Cruz Biotech, Santa Cruz, CA).

Techniques: Expressing, Quantitative RT-PCR

Journal: International Journal of Nanomedicine

Article Title:

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO₂ Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

doi: 10.2147/ijn.s242919

Figure Lengend Snippet: Figure 3 Glucose metabolism, autophagy, and macrophages polarization on the different surfaces. (A, C) Western blot analysis and (D) qPCR analysis of iNOS, Arginase1, p62, Beclin1, LC3, GLUT 1 and GAPDH in macrophages cultured on Ti, TiO2-NTs, Ag@TiO2-NTs, and Ag@TiO2-NTs+STF31. (B) Immunofluorescent staining for GLUT1 in macrophages cultured on Ti, TiO2-NTs, Ag@TiO2-NTs, Ag@TiO2-NTs+STF31. The error bars indicate means ± standard deviations: *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: The expression of proteins was assessed using antibodies targeting TNF-α, TGF-β, CD68, LC3, GLUT1 (Abcam, UK), p-Akt (CST, USA) followed by 50 μL polymerized HRP-conjugated anti-mouse/rabbit IgG International Journal of Nanomedicine 2020:15 submit your manuscript | www.dovepress.com DovePress 2015 In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 45 .1 48 .2 35 .2 02 o n 12 -A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 (ElivisionTM plus Polymer HRP [mouse/rabbit] IHC Kit).

Techniques: Western Blot, Cell Culture, Staining

Journal: International Journal of Nanomedicine

Article Title:

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO₂ Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

doi: 10.2147/ijn.s242919

Figure Lengend Snippet: Figure 5 Fluorescence staining and analysis of LC3 and ROS. (A, C) LC3-Immunofluorescence staining and analysis. (B, D) DCFH-DA probe fluorescence analysis for RAW264.7 macrophages grown on Ti, TiO2-NTs, Ag@TiO2-NTs, Ag@TiO2-NTs+3MA and Ag@TiO2-NTs+3MA+Rapa. (E) the total antioxidant capacity of Ti, TiO2-NTs and Ag@TiO2-NTs that evaluated by the FRAP method.The error bars indicate means ± standard deviations: *p<0.05, and **p<0.01.

Article Snippet: The expression of proteins was assessed using antibodies targeting TNF-α, TGF-β, CD68, LC3, GLUT1 (Abcam, UK), p-Akt (CST, USA) followed by 50 μL polymerized HRP-conjugated anti-mouse/rabbit IgG International Journal of Nanomedicine 2020:15 submit your manuscript | www.dovepress.com DovePress 2015 In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 45 .1 48 .2 35 .2 02 o n 12 -A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 (ElivisionTM plus Polymer HRP [mouse/rabbit] IHC Kit).

Techniques: Fluorescence, Staining

Journal: International Journal of Nanomedicine

Article Title:

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO₂ Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

doi: 10.2147/ijn.s242919

Figure Lengend Snippet: Figure 4 The influences of different surfaces on autophagy and polarization of macrophages. (A, B) Western blot analysis and (C) qPCR analysis of iNOS, Arginase1, p62, Beclin1, LC3 and GAPDH in macrophages cultured on Ti, TiO2-NTs, Ag@TiO2-NTs, Ag@TiO2-NTs+3MA and Ag@TiO2-NTs+3MA+Rapa. The error bars indicate means ± standard deviations: *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: The expression of proteins was assessed using antibodies targeting TNF-α, TGF-β, CD68, LC3, GLUT1 (Abcam, UK), p-Akt (CST, USA) followed by 50 μL polymerized HRP-conjugated anti-mouse/rabbit IgG International Journal of Nanomedicine 2020:15 submit your manuscript | www.dovepress.com DovePress 2015 In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 45 .1 48 .2 35 .2 02 o n 12 -A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 (ElivisionTM plus Polymer HRP [mouse/rabbit] IHC Kit).

Techniques: Western Blot, Cell Culture

Journal: International Journal of Nanomedicine

Article Title:

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO₂ Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

doi: 10.2147/ijn.s242919

Figure Lengend Snippet: Figure 6 The influences of different surfaces on PI3K/Akt pathway. (A) p-Akt immunofluorescence staining and analysis. (B, C) Western blot analysis of p-Akt, Akt, LC3, GLUT 1 and GAPDH, and (D) gene expression analysis of LC3 and GLUT 1 for RAW264.7 macrophages cultured on Ti, TiO2-NTs, Ag@TiO2-NTs, and Ag@TiO2-NTs +740Y-P. The error bars indicate means ± standard deviations: *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: The expression of proteins was assessed using antibodies targeting TNF-α, TGF-β, CD68, LC3, GLUT1 (Abcam, UK), p-Akt (CST, USA) followed by 50 μL polymerized HRP-conjugated anti-mouse/rabbit IgG International Journal of Nanomedicine 2020:15 submit your manuscript | www.dovepress.com DovePress 2015 In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 45 .1 48 .2 35 .2 02 o n 12 -A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 (ElivisionTM plus Polymer HRP [mouse/rabbit] IHC Kit).

Techniques: Staining, Western Blot, Gene Expression, Cell Culture

Journal: International Journal of Nanomedicine

Article Title:

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO₂ Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

doi: 10.2147/ijn.s242919

Figure Lengend Snippet: Figure 7 Osteogenic ability in vitro and in vivo. MC3T3-E1 gene expression for (A) ALP, RUNX2, OCN and OPG. (C) alizarin red staining and (D) ALP for MC3T3-E1 cells. (E) The healing status of bone defect was evaluated by X-ray 2 weeks after surgery. (F) Micro-CTwere used to evaluate new bone formation and (B) the bone volume/tissue volume (BV/TV) ratio. Immunohistochemical staining for (G) TGF-β, (H) TNF-α, (I) LC3, (J) p-Akt, (K) GLUT1and (L) CD68, the surface of Ag@TiO2-NTs significantly promoted the expression of TGF-β, LC3, whereas inhibited the expression of TNF-α, p-Akt, GLUT1and CD68. The error bars indicate means ± standard deviations: *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The expression of proteins was assessed using antibodies targeting TNF-α, TGF-β, CD68, LC3, GLUT1 (Abcam, UK), p-Akt (CST, USA) followed by 50 μL polymerized HRP-conjugated anti-mouse/rabbit IgG International Journal of Nanomedicine 2020:15 submit your manuscript | www.dovepress.com DovePress 2015 In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 45 .1 48 .2 35 .2 02 o n 12 -A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 (ElivisionTM plus Polymer HRP [mouse/rabbit] IHC Kit).

Techniques: In Vitro, In Vivo, Gene Expression, Staining, Immunohistochemical staining, Expressing

Journal: International Journal of Nanomedicine

Article Title:

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO₂ Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

doi: 10.2147/ijn.s242919

Figure Lengend Snippet: Figure 7 Osteogenic ability in vitro and in vivo. MC3T3-E1 gene expression for (A) ALP, RUNX2, OCN and OPG. (C) alizarin red staining and (D) ALP for MC3T3-E1 cells. (E) The healing status of bone defect was evaluated by X-ray 2 weeks after surgery. (F) Micro-CTwere used to evaluate new bone formation and (B) the bone volume/tissue volume (BV/TV) ratio. Immunohistochemical staining for (G) TGF-β, (H) TNF-α, (I) LC3, (J) p-Akt, (K) GLUT1and (L) CD68, the surface of Ag@TiO2-NTs significantly promoted the expression of TGF-β, LC3, whereas inhibited the expression of TNF-α, p-Akt, GLUT1and CD68. The error bars indicate means ± standard deviations: *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The expression of proteins was assessed using antibodies targeting TNF-α, TGF-β, CD68, LC3, GLUT1 (Abcam, UK), p-Akt (CST, USA) followed by 50 μL polymerized HRP-conjugated anti-mouse/rabbit IgG International Journal of Nanomedicine 2020:15 submit your manuscript | www.dovepress.com DovePress 2015 In te rn at io na l J ou rn al o f N an om ed ic in e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 45 .1 48 .2 35 .2 02 o n 12 -A pr -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 (ElivisionTM plus Polymer HRP [mouse/rabbit] IHC Kit).

Techniques: In Vitro, In Vivo, Gene Expression, Staining, Immunohistochemical staining, Expressing